Growth Factor Comparison in Cortical Demineralized Bone Matrix that Demineralized Using Chloric and Acetic Acid

Background: Demineralized Bone Matrix (DBM) is an alternative biomaterial that needs particular acid and a specific time to optimize the growth factors preservation. The best demineralization protocol for preserving in DBM currently unavailable. This study aimed investigate extraction methods using different acids times maintain optimum factors.Methods: vitro experimental laboratory used randomized controlled post-test-only group design. We characterized IGF-1, BMP2, TGF-β content of 1 gram New Zealand White Rabbit cortical bone immersed 0.6 M hydrochloric 0.5 acetic at 3, 6, 9 days. then analyzed differences factor levels each them statistically.Results: IGF-1 were higher demineralized than acid. BMP-2 concentration decreased with highest level was obtained after six days immersion acid.Conclusion: average value Otherwise, BMP 2 better also found reached its peak decreased. These results suggest importance not over-demineralizing factors, further research should be conducted improve processing.